Proton Inventory of Carboxypeptidase Y-Catalyzed Reactions
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چکیده
منابع مشابه
MALT CARBOXYPEPTIDASE CATALYZED AMINOLYSlS REACTIONS
Malt carboxypeptidase catalyzes the formation of peptide bonds using N-benzoyl-arginine esters as acyl components and amino acid amides or amino acid methyl esters as nucleophiles. With seven different nucleophiles yields of 5095% were obtained while no aminolysis was observed with H-Pro-NH2 and H-GIu-a-NH2. The enzyme is easily saturated with nucleophile indicating the formation of a complex b...
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It is demonstrated that carboxypeptidase Y from Saccharomyces cerevisiae can catalyze the formation of peptide bonds using N-acylamino acid esters as substrates and free amino acids or amino acid amides as nucleophiles, The coupling yields observed with free amino acids were max. 60 % for alanine and lysine and they depended strongly on the reaction parameters; viz. pH, temperature and concentr...
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A new carboxypeptidase (carboxypeptidase S) was found in a Saccharomyces cerevisiae strain lacking carboxypeptidase Y (D. H. Wolf and U. Weiser, Eur. J. Biochem. 73:553-556, 1977). Mutants devoid of carboxypeptidase S activity were isolated from a mutant strain that was also deficient in carboxypeptidase Y. Four mutants were analyzed in detail and fell into one complementation group. The defect...
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It is demonstrated that carboxypeptidase Y can be used to exchange the C-terminal alanyl residue of porcine insulin with a threonyl residue, thus forming human insulin. Using threonine amide as nucleophile, the reaction proceeds as a transpeptidation via human insulin amide, des(Ala)a30(Thr-NH2)a30insulin. However, since the amidase activity of carboxypeptidase Y towards this particular insulin...
متن کاملInfluence of the Substrate Structure on Carboxypeptidase Y Catalyzed Peptide Bond Formation
It is shown that for carboxypeptidase Y catalyzed peptide synthesis the coupling yields using peptide esters as substrates and amino acids as nucleophiles are strongly dependent on the C-terminal amino acid residue. A dependence on the length of the acyl donating peptide chain is also observed, indicating the influence of residues other than the one directly involved in acylation of the enzyme....
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ژورنال
عنوان ژورنال: Bulletin of the Chemical Society of Japan
سال: 1986
ISSN: 0009-2673,1348-0634
DOI: 10.1246/bcsj.59.2660